70v is generally used u can play around this value for the better seperation of band.

In our lab, we typically use a voltage range from 70-100 V depending on the concentration of the gel (polarity and area). Hope this helps!

Use voltage in the range of 70-80 V.

For most cases, a voltage ranging from 70-95 volts should work, voltages greater than 100 V might heat your buffer and melt the gel or cause distortion in your DNA bands. You should adjust these values depending on your electrophoresis buffer, the thickness, and concentration of your agarose gel, dimensions of the system's cuvette. There's an empiric formula used to determine the optimum voltage: (length of the matrix)* 5 to 8 V, for example, 10 cm of the gel * 8 V = 80 V.

I suggest you try different values to determine which one gives you better results.

Dear Varugu Lasya Sri ,

For running nucleic acid agarose gel electrophorasis, standard voltage calculation is done by applying 5V/cm of the distance between two electrodes.

You definitely play with this voltage upto 10V/cm according to your product size.