I have used hrp bound sencondary antibody developed with DAB+H2O2+PBS and i am not getting stain it seems like hematoxylin have stained only?

i have used protocol briefly paraffin embedded tissue 5um cut by microtome spread on water and take up on slide with overnight air drying hydration of tissue xylene 10min x2, Ethanol 100, 90, 70,...

07 February 2020 6,973 3 View