How to use a .pdb file as structure template to modeling on Chimera 1.14 ?

Hi, I need to use an own file as a template to modeling on Chimera (using Modeller). When I try to blast my sequence only find the option to query structures available on PDB Web Site. Does...

26 October 2020 2,436 3 View

Can you share your online teaching-learning experience during this pandemic period?

I have started online classes for undergrad, master, and Ph.D. students last January 16 up to the end of May. I have request students to allow recording classes and to discuss the process at the...

14 September 2020 4,466 23 View

How to increase friction between rubber gloves and plate/any item at wet condition?

Its problematic while using household gloves while washing plates, its slippery. I am thinking of why and how to improve the friction.

19 March 2020 5,447 4 View

Why the microstructure of thermally etched 3Y-TZP is abnormal?

These 3 samples are all from TSS (T1=1400, t1=1min, T2=1250 t2=10 min with rate=10degC/min). I did thermal etching for three of them under same batch, i dont know why the images generated got so...

07 May 2019 6,881 3 View

Which are the mechanism that could increase the number of d(GT) repetition in DNA?

How GT dinucleotides repetitions are increased in an microsatellite? Is the replication machinery involved in this phenomenon?

16 January 2017 4,612 2 View

How can I locate the terminator sequences in the pUC19 plasmid?

Hi guys, This might be a dumb question, but I can't locate the terminator sequences for the lacZ gene in the pUC19 plasmid. Apparently none of the maps in the great and mighty Internet (aka...

28 February 2015 8,058 3 View

Do datasets evolve linearly?

If we visualize versions of a dataset in a timeline, is this always a line or are there tree-like bifurcations?

01 April 2014 5,318 3 View

How to determine soil hydraulic characteristics in the laboratory?

I want to estimate groundwater recharge from infiltration in the unsaturated zone with a hydrologic model (MIKE SHE) that requires soil hydraulic characteristics as input parameters?

30 May 2013 9,306 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Charmm/GROMACS Residue 'ZN' not found in residue topology database?

I am trying to do some MD simulations of a protein with zinc ions. I am trying to use a charmm forcefield (I have tried 2017 and 2021) and Gromacs. I get an error when doing pdb2gmx Residue 'ZN'...

12 February 2021 9,367 2 View

Is there any difference between "SEP" as a non standard residue and phohophorylated serine?

I am trying to run a molecular dynamics simulation for a ligand that contains a "SEP" residue. also, I am preparing my files using Charmm-GUI using charmm36 ff, but it says that it will rename my...

31 January 2021 6,321 1 View

How to calculate the error terms in 2-way mixed ANOVA?

30 January 2021 4,684 4 View

Why can I not find all of my residues in model predicted by Swiss-Model?

I am trying to perform homology modeling using the swiss-model but I have found that the swiss-model gives the same number of residues as that of the template. Whereas my sequence has more...

21 January 2021 5,568 3 View

Any resist stripper which can remove thick metal based polymer residue and which is compatible with doped silicon ?

I am looking some resist strippers which can easily remove metal based polymer residue after post dry etch process. I have some candidates below but not sure if they are compatible with doped...

21 January 2021 3,008 3 View

AMBER tleap solvatebox always undervalues the density, why?

I've been searching for an answer to this question for years, and I'm shocked I haven't found any discussion on any forums. Here is an example to provide more details: I am preparing a 450...

20 January 2021 7,561 2 View