Hi Kasi

This depends largely on your own requirements for your mesh and spinning settings. Considering your polymers and the possible solvents you can choose, your working time is the most expensive part, so don't prepare to little amount. My best guess is 10ml will be sufficient, and you will not manage to spin more than 20ml in a day (flow rate ~2ml/h...).

Cheers

Marc

thank you very much Simonet.

The viscosity of the solution is one of the parameter which influence the formation of taylor cone and hence the fibre production. I have spun fibres using PCL in CHCL3 and DCM in the range of 10-15%. I would start with make 7.5, 10 and 12.5% solution and spin a mat for lets says 2 hours each at the flow rate Marc mentions. Scan it using SEM to observed how smooth are the fibres, diameter distribution (image J can be of help ) and check how continuous the fibres are. Formation of nodes, varying of diameter along the axis and breaking indicated lower concentration and hence you might want to go to higher experiments. This optimisation can be done in less then a day, 3 hours for each and 9 hours of exit. Vacuum drying for atlas 2 days before SEM. Hope this helps

hello all

as the Mark said its depend on your own work.

but for more information: when you use PVP in range of 100-150 KDa your best solution viscosity can be reached in 5-15% w/v of PVP and your solvent and you can reach the requirement fiber diameter if it is important for you.

be lucky

Electrospinnibility of polymer solution depends upon nature of polymer solution; concentration and viscosity. It vary with polymer and environments conditions such as temperature, humidity. So based on literature try to prepare nanofibrous film and take its SEM images. Change the concentration and try to optimize.

Hi! Its depends of your solvant and you Pw!  Usually people use PCL 80KDa and for it I prefer to disolve into DCM/DMF. Among 5 to 15% its perfect for electrospinning! Also you may be care of your flow rate and your needle diameter! Good luck.

thanks everyone.