What is the optimal ratio of BAIT:DNA for target capture?

20 November 2015 0 1K Report

I'm trying to capture target genomic loci using biotin-Oligo baits.

I think bait to DNA ration is very important to get accurate results, but I'm not sure how much of them should be used for hybridization. I understand the ratio could be various depending on the experiment, but I just want to get some sense of it.

If anyone has experienced before, would you tell me?

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