We're planning a developmental Golgi study that will look at morphology of neurons at different developmental ages (or PND) in independent groups of young rats. I've successfully stained PND 24 and PND 60 brains in the past using the rapid Golgi stain kit, but never did this on neonate rat brains. I'm aware that incubation time into the Golgi solutions has been scaled back for the smaller brains but I don't have any specific info about how long the neonatal brains has to be kept in for optimal staining w/out background contamination. Any suggestions with references are welcome.
Thanks, Stephane
Hi Emma,
I plan to study both medial frontal cortices like the cingulate and dorsal and ventral striatum. The method I used for golgi staining is the FD Rapid GolgiStain Kit from FDNeurotechologies. Hope this is useful. Let me know what you think and thanks for your advises. Cheers -steph
Great thanks so much Emma I will do exactly that. Happy holiday season and let me know if I can be of any assistance to your work. My area of expertise is neurobehavioral teratology and experimental neuropsychology. I'm very familair with the 5-CSRTT procedures as well. Cheers, Stephane
I think that this article might help you "A modified and highly sensitive Golgi–Cox impregnation of embryonic neurons" by Yoshihisa Koyama and Masaya Tohyama.
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