Stephane A Beaudin

3 Questions 11 Answers 0 Followers

Questions related from Stephane A Beaudin

How long should 100 micron sections be incubated in Cresyl-violet?

I've cell stained 30, 40, and 50 micron sections before but not thicker sections like 100 micron so just wondering if any of you might know the optimal incubation time in Cresyl-violet . I will of...

01 January 1970 225 1 View

What is the best unbiased method for stereology or quantification of immunohistochemistry materials?

I need to known of a non-biased and objective quantification method or software to estimate or count the number of immuno-reactive cells across multiple brain areas and for a number of...

01 January 1970 2,004 11 View

What is the optimal incubation time for Golgi staining of PND 1 rat brains?

We're planning a developmental Golgi study that will look at morphology of neurons at different developmental ages (or PND) in independent groups of young rats. I've successfully stained PND 24...

01 January 1970 6,020 3 View