Does anyone know the TEER value should be constant or it will change over up to 21 days. Does anyone know what are the reasons for increasing or decreasing TEER value.
Thank you for your help.
Hi,
The TEER values of differentiated Caco-2 cells will be influenced by, among others factors, the passage number of the cells, temperature ie., whether you measure TEER immediately after removing the plate from the incubator or allow it to equilibrate at RT, and the culture conditions you have been using (ie. growth medium).
TEER values will increase up to the 21 days of culture as the integrity on the monolayer increases and tight junctions are formed. TEER will begin to plateau at around 21 days of culture (TEER may plateau earlier or later depending on the culture conditions mentioned above).
Literature suggests TEER values of 500 Ohm*cm2 or greater are indicative of fully formed tight junctions. However, the TEER values of the Caco-2s I have been culturing regularly reach up to 1800 Ohms*cm2 after 21 days (I have been using cells of rather high passage number [~p70]). The important part however, is to assay cells once the TEER has plateaued to minimise any experimental variation, and this could take between 21-28 days.
Hope this helps.
Hi,
Condition is different in lab to lab.
The Caco2 cells grown on Costar Transwell inserts (polycarbonate, 12 mm diameter, 0.4 microm pore size) have a TEER of 600-1000 Ohmx cm2 by 14-21 days of culture. If cells are selected with vinblastin TEER can double, and easily reach 2000 Ohmxcm2. See the enclosed paper.
I hope you will get some information .
Hi,
The TEER values of differentiated Caco-2 cells will be influenced by, among others factors, the passage number of the cells, temperature ie., whether you measure TEER immediately after removing the plate from the incubator or allow it to equilibrate at RT, and the culture conditions you have been using (ie. growth medium).
TEER values will increase up to the 21 days of culture as the integrity on the monolayer increases and tight junctions are formed. TEER will begin to plateau at around 21 days of culture (TEER may plateau earlier or later depending on the culture conditions mentioned above).
Literature suggests TEER values of 500 Ohm*cm2 or greater are indicative of fully formed tight junctions. However, the TEER values of the Caco-2s I have been culturing regularly reach up to 1800 Ohms*cm2 after 21 days (I have been using cells of rather high passage number [~p70]). The important part however, is to assay cells once the TEER has plateaued to minimise any experimental variation, and this could take between 21-28 days.
Hope this helps.
Thank you for your answers and all the information you provided.
TEER values are very good up to 10 days of culture. But it was decreased from 12th day of culture in all the wells. And the same was observed till the 21 days. For what reasons it may happened ? What is the solution for it ?
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