How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Has anybody used the Chicken anti-mCherry antibody (ab205402) in immunohistochemistry?

I'm looking for a primary antibody to detect tdTomato in spinal cord slices of mutant mice. I know that the most widely used antibody for this purpose is the mouse anti-RFP from Rockland, but I...

02 February 2021 5,515 3 View

Can you advise reference publications/standards on characterizing animal genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of animal genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of...

31 December 2020 4,924 3 View

Why does chicken primary intestinal epithelial cell could not passage?

05 October 2020 454 3 View

Do the tannic acid-treated sheep RBCs show agglutination with FAdVs (fowl adenoviruses)?

As we know that Adenoviridae indirectly agglutinates the RBCs of chicken, mice, rhesus monkey, and human blood type "O".However, do the tannic acid-treated sheep RBC show agglutination with FAdVs...

17 September 2020 6,630 3 View

Is there any recommended number of birds per replication in poultry research?

In an experiment conducted to evaluate the performance of different chicken breeds, what would be the recommended number of replications per treatment and the number of birds per replication?

15 September 2020 1,618 9 View

Hello Everyone, Can anyone help me in the transfection procedure of plasmid into chicken spermatozoa by electroporation?

Hello everyone, I am doing an experiment in which, the plan of work is to electroporate a clone (plasmid form) into chicken spermatozoa, which will be used further for artificial insemination....

07 September 2020 7,855 2 View

How to statistically analyse feed intake of broilers 90 birds in three groups: factor with two levels?

The effect of two levels of Cl in drinking water of broilers was studied. 30 birds in three groups. weekly feed intake was recorded per treatment. the problem was: no effort was made to divide the...

06 February 2020 7,208 7 View

At what age is the omission of calcium or phosphorus in broiler and pullet feed will result to leg problem in day-old chick ?

I was at a broiler farm recently which had birds 3-day old broiler chick having leg problem. It was attirbuted to feed as DCP was added to the feed and the challenge reduce gradually, but could it...

15 January 2020 664 4 View

There is any way to know the equivalence between an old genome reference assembly and its new version??

28 November 2019 8,768 4 View