How can I measure total phenol and total flavonoid in different extracts?

I have prepared crude extract from the plant material and then fraction it with different solvent. How can I calculate the total flavonoid and total phenol in each fractions. First I extract the...

01 February 2015 1,152 9 View

How does the Comet assay (Single cell electrophoresis) work?

I am working on plant extraction and I want to check the DNA protecting activity of my extracts. How does the comet assay work? Is there any other alternative to comet assay? Can anyone provide or...

03 April 2014 6,526 5 View

What is the basic principle for selecting mobile phase in preparative column chromatography?

I am trying to separate the phytochemicals from the crude extract. What parameters should I consider for the mobile phase selection for column chromatography?

31 December 2013 6,317 10 View

How much concentration of primary and secondary antibody is required to perform the western blot?

09 October 2013 2,432 4 View

How can I select the plant active against diabetes?

On what bases I can select the plant against diabetes? Is there any strategy for selecting plants for specific diseases.

09 October 2013 9,114 6 View

What is the basic difference and what are the applications of immunohistochemistry (ICH), immunoflorescence (IF) and immunoprecepitation (PI)?

I want to know the basic differences between these techniques and their application in molecular biology and pharmacology?

09 October 2013 8,159 1 View

How can I remove chlorophyll from leaf extract without losing other secondary metabolites?

I am working on isolation, purification and characterization of biomolecules from leaf extracts. I have methanolic extract of leaf and I want to remove the chlorophyll from it first.

09 October 2013 7,540 15 View

Could anyone advise me on the process of purification of alkaloids from crude alkaloidal extract?

I have already collected the total alkaloidal extract from crude by acid base extraction. How can I isolate all the alkaloids present in total alkaloidal extract?

08 September 2013 3,490 7 View

Which method is best suited for estimation of Cox II in diabetic rats, ELISA or Western blotting?

Kindly help me to choose the appropriate method for estimation of Cox II in diabetic rats, ELISA or western blotting.

08 September 2013 3,993 20 View

How can I separate Alkaloids, Glycosides, Triterpenoids, Seponins, Flavonoids, etc. from crude extract?

How can I Separate the Alkaloids, glycosides, Triterpenoids, Seponins, Flavonoids from the crude extract without loosing any of the compound by single extraction procedure.

07 August 2013 4,228 20 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View