What is the normal blood leptin level?

Absolute values in Leptin ELISA kit are higher than normal. Does it depend on washing procedure?

I have been using mouse leptin ELISA kit (Catalog number (90030) from crystal chem. I have been doing manual washing between my steps as I didn't have automatic washer instrument. Does this cause...

12 August 2020 4,352 4 View

Best companies to measure RAT hormones and HbA1c?

Good afternoon, Currently my team is performing animal studies with majority of the time using rats of different strains. Our latest experiment was a weight loss study with diet-induced obesity...

14 November 2019 7,139 2 View

Best companies to measure RAT hormones and HbA1c?

Good afternoon, Currently my team is performing animal studies with majority of the time using rats of different strains. Our latest experiment was a weight loss study with diet-induced obesity...

10 November 2019 2,033 2 View

How can I generate a log/log standard curve in R for ELISA analyses?

I carry out ELISA experiments, in which a log/log curve fit is required in order to calculate concentration from O.D. I have used the drc package previously to fit a 4PL model, but this kit...

25 February 2019 8,654 7 View

How can I generate a log/log standard curve in R for ELISA analyses?

I carry out ELISA experiments, in which a log/log curve fit is required in order to calculate concentration from O.D. I have used the drc package previously to fit a 4PL model, but this kit...

01 February 2019 2,938 7 View

What is more appropriate control group in db/db mice model?

We are planning to use db/db mice model in our study. According to articles heterozygous animals are used more often in comparison with WT as the control group. Could you explain what are...

21 January 2019 3,327 3 View

What is more appropriate control group in db/db mice model?

We are planning to use db/db mice model in our study. According to articles heterozygous animals are used more often in comparison with WT as the control group. Could you explain what are...

31 December 2018 2,120 3 View

From where can I find a IR graph or the structure of anti-leptin antibody?

This is mainly to identify the functional groups of the antibody to see what kind of interactions would it show with nanoparticles.

11 December 2018 6,977 0 View

Neuroendocrine models of animal mouse vs rat?

Dear esteemed colleagues, Does anyone have any insight why most of the neuroendocrine studies, i.e hypothalamus-pituitary-peripheral organs axis, are mostly studied in mice? I do know some studies...

22 October 2018 5,908 5 View

Can i measure the leptin hormones in serum of osteoporotic women without fasting?

i read many papers about detection of leptin hormones in women suffering osteoporosis at different ages, but they are in fasting overnight. if possible measure it at normal not fasting state , is...

08 September 2018 4,282 7 View