Not entirely specific, EGTA (a calcium chelator) can usually be used to inhibit granular release of granzymes /perforin in NK/cytotoxic T cells to indicate their involvement in the killing activity of these cells (as one of several mechanisms). Assume you have a specific cell type (non-NK/T/NKT.....) with killing ability. To show whether this ability could be mediated by granular content exocytosis, you pre-treat the effector with EGTA and then expose to the target to evaluate the cytotoxicity (e.g. by chromium release assay). You will most possibly include primary NK cells/cell lines or cytotoxic T cells as positive controls, but what about a negative control cell/cell line? (the best established one!!, forget cells with knocked-out GZM/PRF1 genes, e,g, using CRISPR/Cas9 ). This is what I am interested in, your inputs would be greatly helpful!
Thanks
Jahan
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