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Questions related from Jahangir Abdi
Hi there Can anybody share their experience with flowcytrometric analysis of megakaryocytes in bone marrow samples? Types of markers you used, any set-ups you applied and also whether you used...
02 July 2020 5,813 3 View
Not entirely specific, EGTA (a calcium chelator) can usually be used to inhibit granular release of granzymes /perforin in NK/cytotoxic T cells to indicate their involvement in the killing...
10 September 2019 3,083 0 View
I am going to inject a targeting mAb into xenograft mouse models (IP injection). Here are the questions: 1-How should I determine the dose of the antibody based on the in vitro work (this study is...
26 March 2018 3,033 4 View
Two cell lines are co-cultured in a 96-well plate, cell line A is eGFP-tagged and B is not. To see whether A line is affected by B (after specific treatments), I need to check eGFP signals in a...
26 June 2015 7,519 5 View
Hi guys, I'd like to know what your transfection reagent of choice is for co-transfection of miRNA 3'UTR target clone and miRNA precursor expression clone (both Lentiviral-based vectors) in...
12 April 2015 9,779 4 View
Hi all, I wonder how you measure miRNA in a sample retrieved from a tumor in a xenograft model. After tumor developed, miRNA was injected intratumorarly to look for effects on tumor growth. If you...
10 July 2014 7,158 6 View
I understand JC-1 staining is used by many people to monitor mitochondrial membrane potential in apoptosis induction in a variety of cell systems. I see also different ways of interpreting the...
04 December 2013 4,135 5 View
Does anybody know how to normalize the level of a cytokine to the number of cells treated with a drug? The point is that you have a huge reduction in cytokine production but have to exclude the...
24 April 2013 7,298 6 View
