What is the most used protocol for the transformation of agrobacterium by electroporation?

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Is it recommended to use DNA LoBind tubes for protein extraction - and how, if at all, does it affect retrieval of proteins?

Hi, in our group we are trying to optimize a protein extraction protocol, and I've noticed DNA LoBind tube and DNA LoBind tips are being used. I wanted to know if it is ok to use DNA LoBind for...

30 June 2024 7,751 2 View

Fluorescence spectroscopy: signal from water?

In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...

16 May 2024 6,266 5 View

Can I calculate the high and low spin of the material in Quantum espresso?

I would like to simulate the high and slow spin configuration of a metal oxide. I am using QE for it, but in my research, I found that this kind of calculus is not possible. Or if it is possible,...

15 May 2024 6,497 3 View

Metai ion (zinc2+) docking score calculation?

Is there a way to calculate the docking score of the zinc bound to this amyloid protein https://www.rcsb.org/structure/1ze9 without undergoing the docking procedure which is apparently not...

01 May 2024 8,951 1 View

Is possible to desorbe CO2, N2 or water in ZIF-8 between 30ºC and 60ºC?

Dear all, After checking DSC and TGA analysis for ZIF-8 samples, it was possible to observe an endotermic peak at around 50ºC, using DSC under N2, and a weight loss in TGA at around 38ºC and 125ºC...

04 April 2024 8,460 4 View

¿Qué recursos son necesarios en Servicios Sociales para tratar de eliminar barreras de acceso laboral en personas con discapacidad?

Con este planteamiento se pretende hacer reflexionar a los lectores de los diferentes planes de actuación, medidas y otros tipos de propuestas que pueden ser implantados en los Servicios Sociales...

10 March 2024 1,481 4 View

¿Qué actuaciones faltan para favorecer una garantía de derechos de las personas en situación de dependencia y/o sus cuidadores?

Con este planteamiento se pretende que los lectores tomen conciencia del enorme problema social que supone en la sociedad andaluza que una persona se encuentre en situación de dependencia. De los...

10 March 2024 6,151 3 View

QE with GPU can be installed in a normal CPU system?

I am trying to install QE with GPU. I used this configure instruction: sudo ./configure --with-cuda=/opt/nvidia/hpc_sdk/Linux_x86_64/24.1/cuda/ --with-cuda-runtime=12.02 --with-cuda-cc=61...

26 February 2024 927 2 View

Greetings!, Can anyone recommend a methodology to maintain water stress in maize, at a laboratory level?

I've seen different concepts in various articles, where they mention "maintaining 50% field capacity" or around 30% humidity. But how do I get to that information?

30 January 2024 8,168 2 View

Degassing metallic-Chitosan beads for BET surface analysis, the sample doesnt resist high temperature with vaccum, recomendations?

Hello community, i have trouble degassing Chitosan beads for BET surface analysis, the sample doesnt resist high temperature with vaccum, any recomendations, previous experience analyzing BET on...

16 January 2024 3,430 1 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View

How to prepare a molecule for molecular dynamics calculations?

If I want to calculate molecular dynamics (Gromacs), do I first need to optimize the geometry of the molecule? For example, I first draw the molecule in Avogadro, and then what? I guess I can't...

28 July 2024 673 3 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View