How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

What parameters of quality control would I have to consider for antimicrobial toothpaste?

Hello, my name is Bianca and as a project for university I have assigned the manufacturing of antimicrobial toothpaste- quality control department. I tried to research online but I could not find...

17 January 2021 918 3 View

I am asking about the drug release from minicells, to test the antimicrobial activity of the drug?

I am working on minicells loaded with the drug, I want to test the antimicrobial activity of the drug loaded in minicells using agar diffusion method, many trials didn't produce any zone of...

17 January 2021 7,511 3 View

How to interpret the result of agar well diffusion assay using herbal extract?

I performed the antibacterial activity of isolated herbal extract against Rhodococcus equi and used Erythromycin as a standard drug, ZOI of the plant extracts were 16-20mm and Erythromycin was 28...

17 January 2021 1,746 3 View

How do you determine at what concentration of antimicrobial should you subject the pathogen to?

I am making an antimicrobial extract from fungal metabolite but the fungi are not known. So I don't know at what concentration I should subject them to but what I do know I will test them against...

07 January 2021 897 6 View

Which antimicrobial test will be suitable to determine antimicrobial activity of silver nanoparticles?

There are some journal articles that reported well diffusion method to determine the antimicrobial activity of silver nanoparticles. Meanwhile, some reported disc diffusion method. Which method is...

06 January 2021 7,123 4 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

How yo prepare samples for RNA extraction?

I really need help. I tried finding a protocol on how to prepare samples for gene expression . I am working on Antimicrobial activity of a medicinal plant and I would like to check its effect on...

30 December 2020 7,112 2 View

How many ratio of simplicia powder : water I should add to make the infusion concentration 100%?

I am doing research to test antibacterial activity using plant leaves that I have mashed. How many ratio I should add between the simplicia and water to get 100% concentration in infusion technique?

06 December 2020 1,010 6 View

What is an efficient way of extracting metabolites for fungal isolate from soil for test on its antimicrobial activity?

Hi, I'm finding a method to extract metabolites to evaluate its antimicrobial activity but the identification of the fungi is not done yet. I'm looking for a universal way to extract the...

24 November 2020 2,273 3 View