What is the most effective way to minimize nickel redistribution in plant tissue while performing DMG-staining?

Bien Romulo @Bien-Romulo

07 July 2015 1 280 Report

Some experiments involved freezing the plant tissue with liquid nitrogen but is that the most effective?

Ping Lan

for this, i have no idea

Badges
Science topic

More Bien Romulo's questions See All

Is there a more efficient way to locating the indicated material (below) just to check its validity if at all?

Reference: Author(s): Öztürk, T., Tuncer, G., Özcelik, I. E., & Akçay, N. Publication Year: 2020 Title: The use of virtual reality applications in maritime education and training. Journal or...

21 November 2023 4,993 0 View

What are the ethical considerations surrounding the automation of laboratory testing procedures in Clinical Chemistry?

What are the ethical considerations when it comes to the automation of lab testing in the lab which could result to implications or negative consequences.

23 February 2023 9,849 3 View

Hydrothermal regeneration of hydrochloric acid?

I need additional research materials for my project. I would like to recover hydrochloric acid for reuse in titanium dioxide production.

09 November 2019 5,016 1 View

Hi ... Researchs made by Herail Said that the flat indice of gold could provide informations about its transport in the streams. I don't hear it well?

Herail thinks that with the flat indice of gold, it is possible to estimate the time of transport. How is it doing? Gold has not a particular shape.

03 August 2019 4,654 0 View

Hi! Has anyone here needed to quantify the redox state (NAD / NADH and NADP / NADPH ratios) in mutants of Arabidopsis thaliana in spectrophotometer?

I am with a question of quantification by the Promega NAD / NADH Assay Kit kit and NADP / NADPH Assay Kit ... In the Kit there is no standard to make a dilution curve when there is a known...

24 November 2018 853 0 View

How to input and characterize live oil composition in HYSYS?

Please help me with this case. I have composition report up to C36+ of live oil from PVT lab and crude oil assay from the same sample. My problem is how to input these data in HYSYS. I can input...

21 March 2018 714 3 View

Dears, I am in search of a company that sells sensor type RADFET with a thick layer oxide, sensitive to the range of cGy. Could anyone help me please?

I am part of the scientific team of a mission of young researchers to send a biological mini-lab to the Moon thanks to the company Team Indus. Our experiment requires real-time measurement of...

16 October 2017 5,300 3 View

What type of organic material (except for urine and excrement) may release phosphorous or nitrogen in the directly available form for phytoplankton?

By organic material I mean any kind of material that in theory may be found in the food waste, of both of plant and animal origin. I am trying to estimate what fraction of food waste, that if...

28 June 2016 473 8 View

Does the plant species Breynia cernua exclusively inhabit ultramafic soils?

Breynia cernua is a plant species found in tropical areas including Indonesia, Philippines and Australia. It has been established as a nickel hyperaccumulator in serpentine soils in different...

09 July 2015 3,691 4 View

Who can tell me if there is a mathematical model for the planting of soybeans and corn? Where to have an estimate of production?

12 February 2015 3,631 8 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View