Hi! Has anyone here needed to quantify the redox state (NAD / NADH and NADP / NADPH ratios) in mutants of Arabidopsis thaliana in spectrophotometer?

25 November 2018 0 853 Report

I am with a question of quantification by the Promega NAD / NADH Assay Kit kit and NADP / NADPH Assay Kit ...

In the Kit there is no standard to make a dilution curve when there is a known concentration of the molecule of interest ...

In this case, there is no spectrophotometer quantification of only RLU measurements, right?

Thanks!

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