What is the most digestive enzyme used for lysozyme hydrolysis?

More Dina A. B. Awad's questions See All

How can I extract the mathematical equation from existing Neural Network Model?

There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...

14 July 2024 2,714 3 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

Researchers in the field of AI?

Hi there, this is Dina, a postgraduate student at Warwick University. I am working on my dissertation regarding AI and business. I would like to ask some questions for researchers in this field....

27 June 2024 2,320 2 View

Who is/are the latest theorist of classroom management practices?

latest author latest year concept of the theory

10 May 2024 3,518 2 View

Why light and heavy chains from an antibody don’t appear on a SDS-PAGE gel in reducing condition?

Hello, I have purified a monoclonal antibody from an hybridoma supernatant. When I run different eluted fractions on an SDS-PAGE, I get only one band in reducing condition (the 150 kDa band)....

30 April 2024 931 0 View

Which papers do you believe are crucial for hydrologists to read and thoroughly comprehend in order to establish a solid foundational understanding?

I believe producing knowledge necessitates a thorough grasp of the funcdamentals and hydrological cycle, which may sometimes be underestimated or not fully grasped by researchers. Therefore, I...

07 April 2024 3,788 2 View

Hi guys ,How can I find the nanoparticle of graphene oxide in epoxy powder?

I need the particle size and the shape of graphene oxide in epoxy powder as shown in the following FESEM morphology

08 February 2024 1,335 5 View

How to make Dispase solution 50U/mL?

Hi, I have a problem dissolving dispase into 50 U/mL. I have dispase II powder with enzyme activity of 300.000–360.000 PU/g. Based on catalog information, 1 U is equal to 181 U, but without per...

31 January 2024 8,589 0 View

When fruits ripen, their water content decreases. Is the decrease in fruit water content a cause of ripening or a result of ripening?

In the fruits of the date palm, their water content decreases upon reaching ripen, is this decrease a result of ripening or a cause of ripening

21 January 2024 1,644 1 View

Without job description ?

If there is no job description available for the positions, designing a system and tools to measure and evaluate employee performance can be challenging. However, you can follow these general...

14 January 2024 4,438 1 View

What is the exact procedure and materials for converting from ester to carbolic acid by base hydrolysis??

11 July 2024 4,645 3 View

What could be plastic adherent cells from PBMCs maintained in High Glucose DMEM 15% FBS ?

I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...

26 June 2024 3,664 1 View

Any tips for cell counting in 96-well plate?

Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...

22 June 2024 1,124 5 View

Fibroblast trypsinization in Hanks medium with Ca and Mg?

We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....

28 May 2024 6,052 2 View

Can lysozyme also interact with dextran and agarose?

Hi everyone, I'm a researcher working with proteins and I have a question about performing GPC (size exclusion chromatography). Lysozyme is known to hydrolyze peptidoglycan, which is a component...

25 May 2024 5,979 3 View

Assistant with TUNEL technique for A549 cells, switch from trypsinized cells to adherent cells?

Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...

07 May 2024 4,706 0 View

What are the best conditions for hydrolysis of 4-alkoxyacetanilides using hydazine monhydrate and ammonium iodide?

We aim to devise a technique for estimating 4-alkoxyacetanilides and their analogues by initially transforming them into the respective anilines. These anilines would then undergo diazotization...

29 April 2024 1,650 0 View

Trypsine powder preparation reference?

Hello Please I need urgently a book as reference for trypsine powder dilution to use in tissue culture. With respects

28 April 2024 6,136 0 View

Why can't i download my article to my page?

Use of a maltodextrin-based feed with a lysozyme product to ... Kindly add this article to my profile. Thank you very much Regina

16 April 2024 9,905 0 View

Any advice on how to do cell lysis using osmotic shock?

Hello everyone, I am supervising a master thesis student whose goal is to compare different lysis methods for recombinant protein purification. We are working with E. coli pellet and he is...

08 April 2024 6,240 7 View

Deepti Agrawal

What is the actual intention with lysozyme, is it inhibiting the lysozyme or digesting it and finding the sequence of the same? Once that is known it would be appropriate to answer to your question.

Adam B Shapiro

Trypsin is a good choice for digestion of most proteins because of the abundance of lysine and arginine residues, the selectivity for those residues, and the commercial availability of highly pure enzyme.

Alaa Jabbar Abd Al-Manhel

I agree with Adam answers ..good luck.

Dina A. B. Awad

thanks for all