We have multiple cell lines from different sources. We want to standardize a cell line authentication process for all existing and new incoming cell line.
I am running a coarse DNS case for pipe flow with 2.1 Million cells. My residuals are quite fluctuating as its a fully turbulent annular pipe flow case but its getting statistically converged to a...
29 May 2024 2,063 5 View
I am trying to run a turbulent pipe flow simulation with turbulent Reynolds number of 600, for 15 seconds, the flow is pressure driven due to gravity enforced buoyancy force which gives kinematic...
20 May 2024 5,619 3 View
sodium ion battery is not taking higher current when charging and also has a high voltage drop
06 May 2024 3,182 1 View
In most common turbulence model studies, when considering Reynolds stresses the components of auto-correlation, , , and components of cross-correlation are considered. In the research studies...
02 May 2024 2,776 2 View
I am planning to work on different water bodies of Andhra Pradesh. So I want to purchase a portable water testing kit with multiple parameters. Please suggest me a few best option, which would be...
17 April 2024 7,210 3 View
I need a research topic related to recent trends in GST
16 April 2024 1,805 0 View
I am working on WMLES (wall Modelled LES) for which if I calculate my wall shear stress analytically and want to enforce it as a boundary condition at the wall patch, so that I do not need to...
15 April 2024 9,059 3 View
Is there any specific relation between Re_b (bulk/ Mean Reynolds number) and Re_\tau (friction Reynolds Number). In few of the literature and experimental work I have gone through researchers give...
01 April 2024 861 5 View
This is a code block from nutWallFunction library in OpenFOAM where in, effective kinematic viscosity ($\nut_w$) at the wall is calculated using resolved field(in case of LES)/ mean field(in case...
04 March 2024 9,548 2 View
I am trying to predict the stability of a protein with different SNP. I tried using DUET, Predict SNP and Dynamut. The problem with DUET is that I cannot do double mutation however, it gives fast...
29 February 2024 2,758 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View