Violacein is the purple pigment produced by Chromobacterium violaceum.
Is there any automated system available for counting live vs. dead Caenorhabditis elegans? Microscopic counting is bit tedious and error-prone. MTT assay has been described in literature for...
02 March 2017 5,989 2 View
Is there any correlation between frequency of sound, and its loudness. Here by 'loudness', I mean what we measure with a sound level meter in terms of dB. If this word 'loudness' is replaced with...
02 March 2016 2,936 2 View
We work with pigmented bacteria like Chromobacterium violaceum, Pesudomonas aeruginosa, etc. Sometime they spontaneously loose the property of pigment production. How can we solve this problem? I...
09 October 2015 898 3 View
Pigments produced by bacteria do have some absorbance at the wavelengths (e.g. 625 / 660/ 600 nm) which are used for measurement of microbial growth. How can one minimize the interference from...
03 April 2015 8,975 1 View
We are looking for a simple method which does not require any sophisticated instrumentation. I think there is some method wherein some substance of known molecular weight can be added into the...
06 July 2014 1,664 3 View
Is there any database where one can find information on microbial pigments like violacein, prodigiosin, etc.?
01 February 2014 4,289 0 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...
26 February 2021 9,580 4 View
How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?
23 February 2021 3,053 7 View
Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...
22 February 2021 7,801 3 View
I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...
21 February 2021 3,043 3 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
19 February 2021 472 5 View
