Violacein is the purple pigment produced by Chromobacterium violaceum.
Is there any automated system available for counting live vs. dead Caenorhabditis elegans? Microscopic counting is bit tedious and error-prone. MTT assay has been described in literature for...
02 March 2017 6,099 2 View
Is there any correlation between frequency of sound, and its loudness. Here by 'loudness', I mean what we measure with a sound level meter in terms of dB. If this word 'loudness' is replaced with...
02 March 2016 3,042 2 View
We work with pigmented bacteria like Chromobacterium violaceum, Pesudomonas aeruginosa, etc. Sometime they spontaneously loose the property of pigment production. How can we solve this problem? I...
09 October 2015 1,008 3 View
Pigments produced by bacteria do have some absorbance at the wavelengths (e.g. 625 / 660/ 600 nm) which are used for measurement of microbial growth. How can one minimize the interference from...
03 April 2015 9,140 1 View
We are looking for a simple method which does not require any sophisticated instrumentation. I think there is some method wherein some substance of known molecular weight can be added into the...
06 July 2014 1,781 3 View
Is there any database where one can find information on microbial pigments like violacein, prodigiosin, etc.?
01 February 2014 4,416 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....
31 July 2024 3,843 7 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View
Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...
29 July 2024 1,376 0 View
How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...
28 July 2024 9,165 2 View