Is there any database where one can find information on microbial pigments like violacein, prodigiosin, etc.?
Is there any automated system available for counting live vs. dead Caenorhabditis elegans? Microscopic counting is bit tedious and error-prone. MTT assay has been described in literature for...
02 March 2017 5,989 2 View
Is there any correlation between frequency of sound, and its loudness. Here by 'loudness', I mean what we measure with a sound level meter in terms of dB. If this word 'loudness' is replaced with...
02 March 2016 2,936 2 View
We work with pigmented bacteria like Chromobacterium violaceum, Pesudomonas aeruginosa, etc. Sometime they spontaneously loose the property of pigment production. How can we solve this problem? I...
09 October 2015 898 3 View
Violacein is the purple pigment produced by Chromobacterium violaceum.
03 April 2015 5,003 0 View
Pigments produced by bacteria do have some absorbance at the wavelengths (e.g. 625 / 660/ 600 nm) which are used for measurement of microbial growth. How can one minimize the interference from...
03 April 2015 8,975 1 View
We are looking for a simple method which does not require any sophisticated instrumentation. I think there is some method wherein some substance of known molecular weight can be added into the...
06 July 2014 1,664 3 View
and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?
02 March 2021 557 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...
23 February 2021 1,362 5 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View
Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...
18 February 2021 6,500 3 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?
13 February 2021 3,760 2 View
I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...
09 February 2021 5,155 5 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View