We work with pigmented bacteria like Chromobacterium violaceum, Pesudomonas aeruginosa, etc. Sometime they spontaneously loose the property of pigment production. How can we solve this problem?

I also request suggestion for any special media for pyocyanin production by P. aeruginosa. Does incubation temp have an impact on pigmentation in this organism, as it happens in Serratia.

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