To perform a PCR even a single embryo will work. The question is what is the efficiency of your DNA or mRNA extraction. For PCR you need template DNA in ng only.

Hi, I normally perform my PCR with 20ng/µl DNA and it works. When I extract DNA from a single aphid the average concentration is 100ng/µl. Cheers, Nadine

Hello, the DNA concentration Nadine Gund mentions is enough to sequencing a PCR product, you need 50 ul of 100 ng/ul of PCR product for that purpose.

Thanks everybody! Finally I used 20 embryos and it worked

Hi, how was the concentration? Cheers, Nadine

Hi,

5ng/micro L

Cheers, Maria