What is the minimum concentration of chitosan for GPC?

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Isam Eldin Hussein Elgailani

Dear Sir. Concerning your issue about the minimum concentration of chitosan for the confirmation of molecular weight by GPC. Molecular weight of chitosan can be determined by different techniques such as Gel Permeation Chromatography (GPC), Static Light Scattering (SLS) and intrinsic viscosity measurement. Determination of molecular weight by intrinsic viscosity measurement is a simple method for characterization of chitosan. Different concentrations of chitosan were prepared and measurement was done at room temperature. The flow time data was used to calculate the intrinsic viscosity by extrapolating the reduced viscosity to zero concentration. The value of intrinsic viscosity was then recalculated into the viscosity-average molecular weight using Mark-Houwink equation. The solutions of the chitosan standards and samples were prepared at a concentration of 1mg / cm 3 , using as solvent two aqueous systems: CH 3 COOH 0.10mol / dm 3 - NaCl 0.20mol / dm 3 and CH 3 COOH 0.30mol / dm 3 - CH 3 COONa 0.20mol / dm 3, which were also used as eluents. All solutions were filtered on cellulose acetate membranes of 0.22 mm pore size prior to injection. The calibration curve for each eluent was constructed using the graph of Log M w versus the eluted volume (Ve) for the dextran standards. I think the following below links may help you in your analysis:

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422001000100004

http://www.iaea.org/inis/collection/NCLCollectionStore/_Public/44/122/44122710.pdf

Thanks

Shahrzad Abri

Thanks Amin. Currently, I'm using a 90KDa and 90% DDA with the concentration of 1 mg/ml using acetate buffer as both solvent and mobile phase. I'm having a hard time to get a reasonable peak!

Thanks for your suggestions Amin. Can you please let me know the model of the column you used? I'm having a hard time finding an appropriate column for aqueous state that works well with chitosan.