typical sensitivity range is 5 pg to 1 ng per band. Loading 10 ul puts the concentration at approx 1ng-1ug/ml depending on how pure/how many bands you will have. Volts depends on the length of your gel. Typically run 20-40 V/cm. So a 10 cm gel would use 200V. You want the gel to be warm (~ 40-50°C) during the run to help with denaturation but you dont want it to be hot. Start at the lower end of the range, if it takes too long increase the voltage a little.

Thank you very much for the reply.