Padmapriya Kumar

4 Questions 5 Answers 0 Followers

Questions related from Padmapriya Kumar

How to obtain binding constant and stoichiometry calculation from absorbance/fluorescence data for multiple stoichiometry drug-DNA complex?

which modified benesi-Hildebrand equation can be used?any other method suggestions? I use a quadruplex DNA of 7 bases to study interaction with a drug.

09 September 2015 2,252 0 View

What is the best method to perform a cleavage assay of a 22mer double stranded DNA?

The DNA will cleave into a 10 mer and 12 mer upon treatment with an enzyme. Kindly suggest which labeling is preferable to detect the bands by electrophoresis?

03 March 2013 921 0 View

Is it possible to do PAGE (Polyacrylamide Gel Electrophoresis) for a 22mer DNA sequence without labeling the DNA base?

I want to study binding of an enzyme to the 22mer. The enzyme creates nick in one strand of DNA.

09 September 2012 8,000 0 View

What is the minimum conc and volume, power in Volts required for detection of a 21mer oligonucleotide in denaturing 16% PAGE by silver staining?

I want to run the DNA oligonucleotide sequence and stain it by silver staining. unfortunately I donot have a radiolabelled sequence. kindly help me out.

04 April 2012 6,003 2 View