Does anyone know a coenzyme Q10 extraction protocol for fibroblasts?

Hi, I am looking for a coenzyme Q10 extraction protocol for fibroblasts, in order to be measured by HPLC. Most articles are very vague explaining their procedure. Thank you very much!

05 October 2020 8,245 2 View

It's available the Matlab-code from Traffic Dynamics project ?

I am a master student in Brazil and would like to know if it would be possible to share the Matlab files corresponding to this work (Matlab-code for Traffic Flow Comparison of Roundabouts and...

08 November 2019 4,703 3 View

Were can I find architectural projects for third world countries?

I am working in a arch. project for a comercial subcenter of a city in a third word country but all I find in the internet are examples of first word countries.

28 February 2019 7,058 5 View

How do you detect insulin pathway activation?

Greetings, I was wondering how to detect the activation of the insulin pathway in a cell culture. I thought about quantifying the protein expression of phospho AKT or phospho GSK3 via Western...

15 May 2018 2,388 3 View

How do you detect insulin pathway activation?

Greetings, I was wondering how to detect the activation of the insulin pathway in a cell culture. I thought about quantifying the protein expression of phospho AKT or phospho GSK3 via Western...

04 May 2018 9,410 3 View

Need suggestion for obtain axenic culture of cyanobacteria

I am trying to isolate and purificate cyanobacterial strains from soil sample. Any suggestion will be more than wellcome. Thanks in advance

10 November 2016 9,659 6 View

How can I remove nucleic acid contamination from a cell free protein extract?

06 July 2016 1,377 5 View

Data-base of Spanish Renaissance texts?

I am used to edit Latin Renaissance Poetry. Frequently I do lexical searchs in the Latin Library of Brepols on-line. This let me find the text of the Antiquity which is being imitated, for...

20 March 2016 8,359 5 View

No title

19 November 2015 510 9 View

How can I quantify an uniaxial pressure?

I am performing uniaxial pressure to a tetragonal oxide by reducing one of their cell parameters and relaxing the other two in a computational way. I found that the material is squeezed, suffering...

13 September 2015 9,111 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

Which kind of validity should e established in case we want to conduct a survey using newly developed survey questionnaire?

I am conducting a survey using self developed questionnaire. I have established its face validity and content validity. Some expert reviewer has suggested I must establish the validity by...

11 February 2021 2,077 3 View

Can you remove a publication from me?

I thought it might be mien from decades ago when I did research in math, but it is not mine.

03 February 2021 7,712 4 View

How to apply graphene oxide on an gold electrode?

Dear Fellows, now it is the time to push graphene applications further in terms of easy and low cost production surroundings. Thus I recently dived in the materia and I am facing tons of papers,...

02 February 2021 6,284 2 View