Hi. I would like to know the method of sample preparation of zebrafish embryo for LC-MS for metabolomics analysis. Toxicity assay was done on the zebrafish embryos so after that we would like to apply metabolomics approach to look at the toxicity biomarkers and relevant metabolites.
Have you tried to do a literature search of related type of studies on google, to see how they prepared samples. This would be a starting point for you to maximize procedure for your use.
Thanks Rosa. Yes Wayne, I'll be doing that right away and hopefully be able to come up the best procedure, thanks.
A quick search on Google and I found this paper and you should use as a starting point. If you have issue with protein and phospholips, you can use the new SPE from Phonemenex as a one step cleanup. Please search for Phree and phenomenex. It is faster and more effective than acetonitrile protein precipitation.
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