Kevser Unal

5 Questions 3 Answers 0 Followers

Questions related from Kevser Unal

How do I calculate the protein content from absorbance and standard curve and express as g/100g or percentage?

I did bradford assay and I want to calculate the amount of soluble protein in my glycoprotein sample. I prepared BSA stock of 1mg/ml and did a standard curve ranging from 0.01 to 0.16 mg/ml. I...

07 December 2022 672 0 View

What is the storage condition of freshly prepared OPA reagent?

How do you store OPA reagent made from scratch and how long can it be stored for use?

23 March 2022 1,687 2 View

What is the method of sample preparation for LC-MS?

Hi. I would like to know the method of sample preparation of zebrafish embryo for LC-MS for metabolomics analysis. Toxicity assay was done on the zebrafish embryos so after that we would like to...

21 November 2017 8,586 3 View

How to prepare 4% PFA/PBS for zebrafish embryo ALP staining?

I want to prepare fixation buffer 4% PFA/PBS but I'm confused with the methods I've come across. I tried to follow this one method whereby I dissolved 4g of PFA (merck, for synthesis) in 50 ml of...

10 July 2017 8,333 5 View

Is PHENOMENEX KJ0-4282 Security Guard really a universal HPLC guard cartridge and can it fit all types of HPLC columns?

PHENOMENEX KJ0-4282 Security Guard is said to be a universal HPLC gurad cartridge for analytical columns. However, I have problems when installing it to a WATERS XBridge C18 column 5 μm 4.6 x...

03 September 2015 3,637 10 View