You can follow this protocol for M9 minimal medium http://www.thelabrat.com/protocols/m9minimal.shtml (sterilize the glucose by passing it through a 0,22 um filter before it is added to the diluted M9 salts). We used it for some strains of E.coli. Or you can have a look at the recipe in attached article (used by my colleagues for some other bacteria)

Salmonella strains carries a different type of mutation in the same gene required for histidine biosynthesis.

http://www.answermenu.com/1618/salmonella-different-histidine-biosynthesis-treatment-histidine

Thanks to Zuzana Sramkova  and Mr. Ali Abdil Razzaq Muhammed Noori Aldallal...

Thanks Jiansheng Huang .....

Minimal Medium

M9 minimal salts solution (5X concentrate) :

1. To 800mL of distilled/deionized water add:

· 64g Na2HPO4-7H2O

· 15g KH2PO4

· 2.5g NaCl

· 5.0g NH4Cl

Make to 1 liter with dH20

Sterilize by autoclaving (or filter sterilization if autoclave is not available.)

2. Preparation of (1 M MgSO4):

To 100 mL distilled/deionized water add:

· 24.65 g MgSO4•7H2O

3. Preparation of 40% glucose (w/v):

To 100 mL distilled/deionized water add:

· 40g Glucose

v Add glucose to stirring water in beaker; Do not attempt to add water to GLUCOSE!

4. Preparation of (1M CaCl2):

To 100 mL distilled/deionized water add:

· 147.014g CaCl2·2dH20

Make to 1 litre with dH20

Method for preparation of (1L of media) minimal medium:

· 200mL 5X M9 salts solution

· 800mL of distilled water

Ø Add 15g of agar media if agar plates are to be poured.

After autoclaving, swirl to mix evenly and "temper" at room temperature (until you can place your hand on the flask for 2 second) then add:

· 2mL of 1M MgSO4 solution

· 0.1mL of 1M CaCl2 solution

· 20mL of 20% glucose