What is the method for Fmoc deprotection if compound is water soluble ?

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Supriya Dey

If u can add H20-DMSO mixture then it need only 120 oC heating.

Ramin Zibaseresht

Hi Surajit,

I have read many papers in which deprotection using 25 % piperidine /DMF, have been reported... haven't seen other reports in water. However, since DMF is miscible in water, you can try a water solution of your oligo in DMF.

Ramin

Surajit Sinha

I would like to thank Supriya, Rafik and Ramin for your answer. In water medium, 20 to 30% piperidine worked.

That sounds great.

Good luck.

Aleksei Smirnov

Supriya Dey what is the H2O:DMSO ratio and incubation time?