What is the method for determining phosphatase activity of bacteria in solid culture medium?

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Anoop Kumar Srivastava

Please have a look at enclosed PDFs...

Kulvir Singh

Dear Mostafa

Please find attached the relevant content.I hope this will give you some working clues.

Good luck.

J. C. Tarafdar

Analysis of phosphatases involves spectrophotometric estimation of the p-nitrophenol released when known amount of culture is incubated with buffered sodium p-nitrophenol phosphate solution and toluene. The producer used to extract the p-nitrophenol released by phosphatase activity develops the stable color used to estimate this phenol gives quantitative recovery of p-nitrophenol added to culture.

Hanaa Ahmed Abokora

please have a look in pdf

Vol.8, No.9, pp 100-113, 2015