What is the mechanistic action of crayfish diet (animal protein) on serum biochemical and heamatological parameters of animal fed same diet?

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

Which mesostigmatan mites from black soldier fly farm?

I have aquired some mites which have been isolated from feeding trays at a black soldier fly farm. These mesostigmatans are not my primary field of interest, although they are very beautiful. Can...

13 July 2024 10,036 5 View

The HCP removal rate is lower than usual in concentration step, why?

Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...

11 July 2024 5,380 0 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

How am I supposed to find justifications for the unusual fatty acid profile in broiler diet?

I've obtained a fatty acid profile of broiler diet comprised of SFA 48 to 93%, MUFA (22 to 40%), PUFA (non determined to 10.4%. The PUFA composition is mainly n-6 and almost no value for n-3. The...

08 July 2024 9,165 4 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Chinaza Godswill Awuchi

Check https://www.mdpi.com/2076-2615/11/6/1761/htm

https://www.sciencedirect.com/science/article/pii/S0147651321000075

Also check https://fas.biomedcentral.com/articles/10.1186/s41240-019-0117-4

http://www.fao.org/3/i1142e/i1142e.pdf

Kiprotich Kiptum

Also check on this paper

https://www.ajol.info/index.php/ajbr/article/view/126143/115671