I want to know about the antibodies capability to pass through tissues, especially blood vessel wall tissue.
Antibodies are transported across epithelial cells by the Fc-receptor FcRn
Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...
28 February 2021 364 3 View
Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...
09 February 2021 3,595 8 View
In my research related to monitoring the cow behavior with help of sensors I have a permanent and principal problem with the data for model validation. I tried to find some available open-source...
26 December 2020 8,197 2 View
Hello, For a project which studies how teens deal with social media and their interfaces, I'm interested in so-called "activity theory" (also know as cultural-historical activity theory). I know...
21 October 2020 2,364 4 View
Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?
07 October 2020 739 3 View
I am trying to design a vector as compact as posible to test DNA transcriptional enhancer and/or repressor activity, I already have a compact bacbone (pICOz vector), minimal promoter, translation...
22 August 2020 1,083 1 View
Hi everybody! I need to amplify phage DNA so as to be able to produce restriction profiles using different restriction endonucleases. Can anyone recommend which primers should I use? I know random...
06 August 2020 1,194 3 View
My current research is about SPC and I'm interested in tuning strategies for these types of predictive control approaches. The cost function is on the photo and the tuning parameters are Np...
23 July 2020 1,176 1 View
On the role of aluminum in TWIP steels
22 July 2020 5,670 4 View
There are some theories, as Pelgrum and Law (2003) suggested that it is necessary to make a switch between the teacher and the student roles. I mean, the student should be also the active role in...
15 July 2020 5,169 15 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View