Does the ester group act as donor or acceptor?
Please rewrite your question and give more infos about the ester compounds.
- According to March J. (Advanced Organic Chemistry/Third Ed./ 1985 /Wiley Interscience , you c an have esters of organic and inorganic acids.
- Among the organic esters, you have the aliphatic esters and the aromatic esters.
Chemical reaction of esters:
Esters react by nucleophilic substitution at the carboxyl group (sn2 reaction p.e.)
Ester + X (X can be OH- or NH3 . please read the chapter in March ´s book.
Concerning the fluorescence of aromatic esters (with benzene ring) like :
dimethyl 2,5-diaminobenzene-1,4-dicarboxylate give a YELLOW fluorescence in diethylether.
With the introduction of further methylcarboxylate( 2 at the free positions of this molecule) the fluorescence is RED in diethylether.
The esterification of fluorochromes makes them lipophilic prior to an introduction in membranes. Scission to free acid occurs in the cells.
Whether or how the ester group acts can be answered when you rewrite your question and give more infos about the choosed ester compounds.
JRG
Sir the triterpenoid esters precisely .I would like to thank you so much for your explanations
I have a follow-up question. This ester, 7-Hydroxy-6-methoxy-2H-1-benzopyran-2-one is fluorescent at neutral pH and it loses fluorescence at acidic pH and also upon oxidation. Can someone please explain the underlying mechanism
Please refer to following article. Let me know if you need further explanation Chandan kumar Gautam
Article Fluorescence of Scopoletin Including its Photoacidity and La...
I wanted to study the conformational changes taking place in gamma D crystallin (eye lens protein) at low pH via NMR. What would be the preferred solvent requirements for this particular protein...
31 December 2020 2,103 4 View
I have to simulate multipath fading channels and add randomness to it.
21 September 2018 6,377 2 View
Anthocyanins in plant extracts
13 September 2015 1,366 4 View
I need to prepare the setup for Ca test for WVTR measurement. I am not sure how to deposit calcium on my glass substrate . We don't have ALD.
02 October 2014 3,057 2 View
I used 4 month old stored DNA @ -20 deg C. I got good PCR bands but not good result for pyrosequencing. Would it help if I store @ - 80 deg C and make aliquots of that?
04 May 2014 8,606 3 View
01 January 1970 4,548 3 View
I am using ICC to stain some nuclear proteins in muscle-derived stem cells. I'm currently using a HRP-conjugated secondary and DAB substrate (From Biogenex) I would like to avoid haematoxylin as a...
01 January 1970 4,694 3 View
I am working on a small dataset with 22 observations and using 30 variables under eight dimensions to prepare an index. I am using factor analysis for the same. But the condition for factor...
01 January 1970 7,418 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View
Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...
20 February 2021 1,291 3 View
I'm using blast furnace slag for stabilizing subgrade soil.
17 February 2021 7,333 3 View