Sometimes we observed a single excitation with multiple emission patterns in EEM, and sometimes otherwise. What is the meaning of each phenomenon? Is that means several compounds exist with similar molecular structure in the sample? Then, what is the variation of the compound molecular structure that makes it different in excitation (with single emission) and different in emission wavelength (with single excitation)?
How to distinguish from EEM data: investigate emission & exciation spectra for all fund emissions.
- If several emissions have differing excitation spectra, there are several species present in ground state with distinct absorbance spectra
- If several emissions have same excitation spectra, it is likely there is only one species present, or else several species with very similar absorbance spectra. If it is one absorbing species, excitation state transformations are leading to differing emissions.
"Several species" can still mean it is the same compound, but present in differing forms, which can be e.g. prototropic forms, depending on pH value.
Generally: if there is one species present with no excitation state transformations taking place (which is the "normal" thing), you receive the same emission spectrum for _all_ excitation wavelengths.
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