In free-flow electrophoresis tray is it necessary that the electrodes be in touch with liquids (buffers) or can they be designed behind the chamber?

I am going to fabricate a free flow electrophoresis (FFE) chamber. The chamber will contains the sample plus buffers (electrolytes). In capillary electrophoresis, IEF or gel-based electrophoresis...

05 June 2019 6,753 4 View

Can any body explain me what are the dipole moment legends (mentioned in figure) in .xvg file of Gromacs g-dipoles command ?

04 May 2017 7,612 1 View

How can simulate electromagnetic wave effects on protein structure in silico (with gromacs)?

I want to study effects of electromagnetic (EM) wave (UV-Vis) on the secondary and tertiary structure of a protein in silico. The protein structural changes are proved by experimental results...

09 October 2016 6,659 0 View

Can I use an amicon millipore centrifugal filter instead of dialysis bag?

08 September 2014 2,775 13 View

What is the most quick and easiest test to determine a protein purity after purification?

I need to know what is the most quick and easiest method/test to determine a protein purity after purification? I am tired from preparation and running the 1D SDS PAGE...

08 September 2014 1,912 3 View

Can you recommend a protocol for recharging Ni-NTA aGAROSE resin?

07 August 2014 8,018 3 View

How I could determine the extinction coefficient (Molar absorptivity) of my protein experimentaly?

How I could determine the extinction coefficient (Molar absorptivity) of my protein experimentaly? Is it okey if I calculate the slop of Absorption vs Concentration? for this I must prepare some...

07 August 2014 4,595 6 View

How I can wash a crystal in order to SDS PAGE analysis?

I Have a Crystal in my drop. my I don't know really what is it, a protein or salt or contaminant ! I want to apply SDS PAGE to understand it. But what is the best way to handling the crystal and...

07 August 2014 1,458 8 View

How could investigate the protein crystal quality in post-crystallization (pre-diffraction) stage?

We have not synchrotron center in our region, so it is important for us to obtain certainty about the protein crystals quality before x-ray diffraction.

04 May 2014 3,330 3 View

Plexiglass (poly methyl methacrylate) scraps recycling

I have accessibility to a cheap source of plexiglass scraps. I am thinking to a recycling projects and producing new products based on PMMA recycling. What is your suggestion for PMMA recycling?...

01 January 1970 2,947 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View