Zaiddodine Pashandi

11 Questions 12 Answers 0 Followers

Questions related from Zaiddodine Pashandi

In free-flow electrophoresis tray is it necessary that the electrodes be in touch with liquids (buffers) or can they be designed behind the chamber?

I am going to fabricate a free flow electrophoresis (FFE) chamber. The chamber will contains the sample plus buffers (electrolytes). In capillary electrophoresis, IEF or gel-based electrophoresis...

06 June 2019 6,838 4 View

Can any body explain me what are the dipole moment legends (mentioned in figure) in .xvg file of Gromacs g-dipoles command ?

what are the means and differences of "< |M|\S2\N >" , "< |M| >\S2\N" , "< |M|\S2\N > - < |M| >\S2\N" and "< |M| >\S2\N / < |M|\S2\N >" in .xvg file of...

05 May 2017 7,666 1 View

How can simulate electromagnetic wave effects on protein structure in silico (with gromacs)?

I want to study effects of electromagnetic (EM) wave (UV-Vis) on the secondary and tertiary structure of a protein in silico. The protein structural changes are proved by experimental results...

10 October 2016 6,726 0 View

What is the most quick and easiest test to determine a protein purity after purification?

I need to know what is the most quick and easiest method/test to determine a protein purity after purification? I am tired from preparation and running the 1D SDS PAGE...

09 September 2014 1,960 3 View

Can I use an amicon millipore centrifugal filter instead of dialysis bag?

I have a 24KD protein Eluted from Ni-NTA agarose by Elution buffer (NaH2Po4 50mM/ NaCl 300mM/ Immidazol 300mM/ pH= 8). Routinely, we dialysis this protein with dialysis bag (2 hr* 1liter dialysis...

09 September 2014 2,837 13 View

How I can wash a crystal in order to SDS PAGE analysis?

I Have a Crystal in my drop. my I don't know really what is it, a protein or salt or contaminant ! I want to apply SDS PAGE to understand it. But what is the best way to handling the crystal and...

08 August 2014 1,567 8 View

Can you recommend a protocol for recharging Ni-NTA aGAROSE resin?

I use Ni-NTA Agarose resin to purification a protein with his-tag tail. As I use it multiple times, it seem that it has lost the efficiency. I want to recharge it to become refreshed. What is the...

08 August 2014 8,067 3 View

How I could determine the extinction coefficient (Molar absorptivity) of my protein experimentaly?

How I could determine the extinction coefficient (Molar absorptivity) of my protein experimentaly? Is it okey if I calculate the slop of Absorption vs Concentration? for this I must prepare some...

08 August 2014 4,639 6 View

What is the mean of "The protein was homogeneous according to LC-Electrospray Ionization Mass Spectrometry."

There is a common sentence "The protein was homogeneous according to LC-Electrospray Ionization Mass Spectrometry" in the crystallography papers. Do they prepare the protein sample as it used for...

07 July 2014 1,154 1 View

How could investigate the protein crystal quality in post-crystallization (pre-diffraction) stage?

We have not synchrotron center in our region, so it is important for us to obtain certainty about the protein crystals quality before x-ray diffraction.

05 May 2014 3,382 3 View

Plexiglass (poly methyl methacrylate) scraps recycling

I have accessibility to a cheap source of plexiglass scraps. I am thinking to a recycling projects and producing new products based on PMMA recycling. What is your suggestion for PMMA recycling?...

01 January 1970 2,994 8 View