In free-flow electrophoresis tray is it necessary that the electrodes be in touch with liquids (buffers) or can they be designed behind the chamber?

I am going to fabricate a free flow electrophoresis (FFE) chamber. The chamber will contains the sample plus buffers (electrolytes). In capillary electrophoresis, IEF or gel-based electrophoresis...

05 June 2019 6,753 4 View

Can any body explain me what are the dipole moment legends (mentioned in figure) in .xvg file of Gromacs g-dipoles command ?

04 May 2017 7,612 1 View

How can simulate electromagnetic wave effects on protein structure in silico (with gromacs)?

I want to study effects of electromagnetic (EM) wave (UV-Vis) on the secondary and tertiary structure of a protein in silico. The protein structural changes are proved by experimental results...

09 October 2016 6,659 0 View

Can I use an amicon millipore centrifugal filter instead of dialysis bag?

08 September 2014 2,775 13 View

What is the most quick and easiest test to determine a protein purity after purification?

I need to know what is the most quick and easiest method/test to determine a protein purity after purification? I am tired from preparation and running the 1D SDS PAGE...

08 September 2014 1,912 3 View

How I could determine the extinction coefficient (Molar absorptivity) of my protein experimentaly?

How I could determine the extinction coefficient (Molar absorptivity) of my protein experimentaly? Is it okey if I calculate the slop of Absorption vs Concentration? for this I must prepare some...

07 August 2014 4,595 6 View

How I can wash a crystal in order to SDS PAGE analysis?

I Have a Crystal in my drop. my I don't know really what is it, a protein or salt or contaminant ! I want to apply SDS PAGE to understand it. But what is the best way to handling the crystal and...

07 August 2014 1,458 8 View

What is the mean of "The protein was homogeneous according to LC-Electrospray Ionization Mass Spectrometry."

There is a common sentence "The protein was homogeneous according to LC-Electrospray Ionization Mass Spectrometry" in the crystallography papers. Do they prepare the protein sample as it used for...

06 July 2014 1,106 1 View

How could investigate the protein crystal quality in post-crystallization (pre-diffraction) stage?

We have not synchrotron center in our region, so it is important for us to obtain certainty about the protein crystals quality before x-ray diffraction.

04 May 2014 3,330 3 View

Plexiglass (poly methyl methacrylate) scraps recycling

I have accessibility to a cheap source of plexiglass scraps. I am thinking to a recycling projects and producing new products based on PMMA recycling. What is your suggestion for PMMA recycling?...

01 January 1970 2,947 8 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View