Please help me with the best weight of healthy callus obtained from mature embryo of wheat..my 5 week old callus are 100mg..
The growth of callus will vary depending on growth regulator you have used as well as the type of explant taken for culture.
Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...
23 February 2021 9,956 3 View
Hello, For now, I am following the integrated technique to derive the mass from the smaller scale (microstructure). Is there any better approach to get the mass of a given macro-structure from...
16 January 2021 4,792 1 View
I'm planning to write a Review article, to make it more worthy and easy to understand for the targeted audience, I'm thinking to add some signalling pathways, and unfortunately, I have no idea how...
30 December 2020 6,395 2 View
Dear researchers, I need this research paper "Similarity solutions of the boundary-layer equations for a stretching wall by Banks, W. H. H (Bristol, University, Bristol, England)." If anyone...
07 December 2020 2,157 1 View
Can anyone tell me how to simulate noise of CMOS image sensors, such as thermal noise and flicker noise with sentaurus TCAD?
09 November 2020 9,616 4 View
In the simulation tutorial of the aeroacoustics around a cylinder by ANSYS, recievers are placed at a location where mesh isn't present, how does the solver predict the acoustics at that location...
16 October 2020 3,368 5 View
Im new to quantum espresso and i wanted to detect the d33 value of a pervoskite crystal. Apart from this I would be nice to obtain the PE loop also. can anyone share the input files for such a...
14 September 2020 6,590 2 View
Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...
10 September 2020 3,767 3 View
07 September 2020 1,318 4 View
In my lab, we are using Toluene for extraction of PAH from biochar (byproduct of pyrolysis). There are numerous samples to measure. Therefore, to make it economic can we distill toluene from the...
03 September 2020 6,111 5 View
I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...
18 February 2021 6,494 4 View
Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...
17 February 2021 3,368 2 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...
12 February 2021 294 4 View
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
critical range for Mn in soils is 2.5-4.5 ppm for all plants as an average, but what is the exact amount of DTPA extractable Mn in soil for wheat crop
04 February 2021 4,008 4 View
The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...
03 February 2021 4,753 7 View
I'm trying to whole mount in situ hybridization in fish embryos. I fixed fish embryos in 4% PFA in PBS for 2days. And I found some sharp brown spots in embryo. I've never found that spots...
12 January 2021 2,722 2 View
how to prepare wheat grain sample for amino acid analysis through UV-HPLC, and also amino acid standards for HPLC , I will be very very thank full.
10 December 2020 1,207 3 View
I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...
07 December 2020 7,744 3 View