What are Extensors of space and time in Generalized Thermodynamics?

Generalized Thermodynamics, which formulates the most universals laws of nature, assumes separation of all fields variables into conservable 'extensors' E and 'intensials' P linked by energy dU =...

20 October 2020 8,117 4 View

Antibodies against gram-negative peptidoglycan?

Hi everyone! I am trying to find commercially available antibodies that react with peptidoglycan from gram-negative bacteria. So far I have only found anti-PG mAb 3F6B3 (e.g. here...

11 September 2019 3,950 9 View

Why does the failure curve have a steep descent at its beginning?

It does not depend on the nature of the object: from a rivet to a human. Why does any kind of life and man-made things obey one law, according to which the entity, newly arrived in the world, has...

31 July 2019 5,215 6 View

Where can I see the formula for determining the minimum sample size for estimating the average random variable distributed according to some law?

I examine the distribution of some random variables in order to estimate their average value. The sample size is 116 values. Known expectation, variance and other statistical characteristics. It...

03 February 2019 8,983 3 View

Is it possible to determine quantitative composition of mixture by intergation of TIC chromatogram?

I'd be grateful if you will provide me some literature data about quantitative analysis of mixtures using TIC chromatogrames.

29 January 2019 8,652 13 View

What strain to use in cloning with araBAD promoter and gene of interest(1000bp) that is complementary to the region on the same plasmid?

I am trying to insert into PBR322 araBAD promoter with my gene of interest(it should be inducible). Could you please tell wether I could use DH5alpha strain as I was told it lacks arabinose...

28 January 2019 3,370 4 View

Who knows how to change computer atomic operations (a+b,a*b...) to new ones?

I seek advice from computer hardware/software specialists how to change atomic operations (a+b,a*b,a-b,a/b) to other ones on a hardware or core software/bios/OS levels. For example, a+b should be...

20 March 2018 2,001 3 View

How can i confirm presence of crystalline phase inside the glass volume?

If the volume fraction of crystals is so small that XRD doesn't show it. Any method beside HR TEM?

10 July 2017 3,027 10 View

What are the typical values for dislocation velocity, density of dislocations, and/or strain rate in plastically deformed graphene at room temperature?

...

23 June 2013 757 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View