What is the maximum value of minimum inhibitory concentration (MIC) for a potentially therapeutic antibacterial agent?

More Sergei Grishin's questions See All

What does it mean high-precision determining random variable?

Hello everyone! As you understand, high-precision positioning using global navigation satellite systems or simply high-precision determination of a random variable. At what point does your...

11 February 2024 6,785 7 View

Where can I get the dataset of the network of passenger flows on urban public transport?

The number of passengers entering and exiting at each stop is the basis for optimizing bus operations in cities. Currently, there are many technical solutions available to calculate passenger...

25 December 2023 2,348 0 View

Can anyone suggest why the D283 medulloblastoma cell line did not attach after thawing?

Can anyone suggest why the D283 medulloblastoma cell line did not attach after thawing? What changes can be made to the dish surface or culture medium to solve this?

30 November 2023 3,338 2 View

Does anyone know where to find polymer-solvent Flory-Huggins interaction parameters for PEG 400 and 1,4-dioxane?

I am searching for polymer-solvent Flory-Huggins interaction parameters for PEG 400 and 1,4-dioxane to determine the cross-linking density of polymer network containing PEG 400 using 1,4-dioxane...

18 September 2023 8,258 2 View

Are there resources for identifying the circuit elements of an FPGA?

I have been trying to find a resource to identify the circuit element structures (from an optical micrograph) of delidded/decapsulated electronic devices, including the imaged Xilinx Arty A7 FPGA....

13 September 2023 2,765 0 View

How do I get CD25 expression on Jurkat cells?

After using flow cytometry to check early activation of Jurkat cells by estimating CD69 expression after 4 hours of incubation with CD3/CD28 ABs, I need to do the same for the late activation. So...

10 August 2023 5,708 1 View

Can anyone recommend a hydraulic lab that has a zero slope flume?

In fluid mechanics, the theory of steady and uniform fluid motion does not allow such a flow in a horizontal channel. But such a flow is possible from the point of view of the theory of energy...

19 April 2023 7,170 2 View

How is the process of interaction between the authorities, the operator and passengers in the service of passengers by public transport organized?

Public transport routes and timetables in citys are determined through a collaborative effort between local transport authorities and public transport companies. Local authorities are responsible...

04 April 2023 9,104 1 View

Can I prove that my metallic specimen has no carbon contamination by using SEM/EDS analysis?

I fabricated a Ni-based alloy specimen with C content in raw material less than 0.1%. Theoretically, there were added some contaminations during the manufacturing process, but I'm unsure which...

21 February 2023 5,754 4 View

How to optimize the public transport network?

In my city, passengers are transported by buses. The number of routes is very large. Many routes overlap. As a result, the costs of public transport from the city budget are high. The task is to...

24 January 2023 8,065 6 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Abhijeet Singh

There is no general criteria, it would depend on the type of compound.

Seshagiribabu Yagani

It's depends on different drugs and organism. I have seen till now maximum number 256

Dhuha Jasim

The value of ( MIC ) determined according by species of microorganisms and there's no stable value for it

Neerja Shrivastava

It would be depend on compound