Hello All,

Background:

I am currently planning some fluorescence quenching experiments for a purified membrane protein that will be reconstituted into proteoliposomes. The quencher I will be using first will be acrylamide and based on previous experiments in detergent micelles I'd like to go up to around 900mM if possible. However, I would like to minimize the volume change in my sample over the course of the fluorescence run and therefore would like to use the highest concentration of acrylamide possible but I am concerned about the possibility of the high concentration acrylamide I am adding disrupting my liposomes.

Question:

So I was wondering if anyone knows and/or can point me towards a reference that discusses the maximum stock concentration of acrylamide that can be used for fluorescence quenching studies of membrane proteins reconstituted into proteoliposomes?

Thanks in advance for your assistance