Tyler Auld

2 Questions 2 Answers 0 Followers

Questions related from Tyler Auld

What is the Maximum Acrylamide Concentration for Fluorescence Quenching of a Protein Reconstituted into Proteoliposomes?

Hello All, Background: I am currently planning some fluorescence quenching experiments for a purified membrane protein that will be reconstituted into proteoliposomes. The quencher I will be using...

28 January 2015 3,321 2 View

Why might a mutant protein with all Tryptophan residues substituted have the same absorbance at 295nm and emission (excitation@ 295nm) relative to wt?

I am running fluorescence spectroscopy on a protein with two endogenous tryptophan residues and comparing the fluorescence with that of a site-directed mutant of the same protein, in which both...

19 June 2014 3,614 13 View