03 April 2025 1 6K Report

Please share the protocol in details for carrying out glucose estimation using DNS method.

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Cyclization should be performed before mutation or after mutation of a linear peptide sequence by in-silico method?

I have a doubt on in-silico modeling. I want to cyclize a linear peptide sequence by in silico. Which is more favorable method I don't understand. There is 2 pathways- 1. I can cyclize a linear...

12 June 2024 2,449 2 View

What does the SST field represent in the GFS forecast/ analysis?

What does the SST field represent in the GFS forecast/ analysis? For example, in the ERA5 reanalysis product, the SST is a foundation SST taken from the OSTIA. Similarly, what is the SST in GFS?...

11 June 2024 8,752 0 View

What cDNA dilution can be used for qPCR reaction?

I have obtained 100 ul of pre-amplified cDNA using the SSIV SINGLE CELL PRE-AMP KIT. Now i want to make a dilution because my samples are limited and i cannot directly use the cDNA. So what...

22 May 2024 6,017 3 View

How can I calculate average dG MM/GBSA energy from selected frames of MD trajectories?

I need to calculate dG MM/GBSA energy through the MD simulation trajectories. From RMSD values I selected last 400 frames from MD trajectories. I ran thermal_mmgbsa.py script, but it calculates dG...

22 May 2024 10,076 5 View

Is it necessary to change the medium every 24 hours after drug treatment in cell line study and provide fresh drug to the cell?

I am keeping my cell line for drug treatment and monitoring its effect at 24 hrs, 48 hrs and 72 hrs? Should I change the medium every 24 hrs and give fresh drug treatment or just leave the medium...

01 May 2024 10,011 3 View

Can anyone explain the N/P charge ratio of protein DNA complex during LLPS formation?

Protein:DNA charge ratio plays an important role in forming condensates. In most cases the N/P ratio ~1 is favored. However how this ratio depends on the concentration of the protein is not...

20 April 2024 4,753 0 View

How BLUE ECONOMY can upgrade the economic profile of the nations?

The "blue economy" refers to the sustainable use of ocean resources for economic growth, improved livelihoods, and jobs while preserving the health of ocean ecosystems. It encompasses a wide range...

19 April 2024 7,456 2 View

What could be the possible reason for this different microstructure?

I've prepared the following ceramics: Sample ID = Composition LMT = Li2Mg3TiO6 LMCT = Li2(Mg2.88Ca0.12)TiO6 LMCT-MN = Li2(Mg2.88Ca0.12)(Ti0.95(Mg1/3Nb2/3)0.05)O6 LMT-MN =...

07 April 2024 4,717 2 View

How AI can assist in the progress of Oceanography?

AI has immense potential to address various aspects of climate change by enhancing our understanding of the climate system, optimizing resource usage, and developing innovative solutions. Here are...

26 March 2024 958 1 View

How to homogenize gizzard tissue using Ultrasonic cell crusher?

I want to extract RNA from chicken gizzard tissue. But currently I don't have a homogenizer. What parameters should I use, like the power, Ton, Toff, total time etc, to effectively homogenize the...

29 February 2024 8,155 0 View

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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How to prediction 2D to 3D aptamer?

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What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

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Can type 2 diabetes be reversed at any age through proper diet and exercise?

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07 July 2024 8,745 2 View

Hello all Can we use different concentrations of lemon or orange extract to grow flowers in ornamental trees and shrubs?

I need more pdf on this topic

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Can someone help me get this article?

..?

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Litopenaeus vannamei post-larvae for fatty acid and amino acid profile?

For fatty acid and amino acid profile analysis in Litopenaeus vannamei post-larvae (1-1.5 gr), is it necessary to remove the head and peel? or it's possible to use whole body including head, peel...

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Is there any technique which can help in determining sugar concentration in water?

"I want to determine the concentration of a series of sugar solutions. I tried to use refractometry, but the concentration is very low and the difference between the refractive index (RI) values...

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Is there an HSQC sequnce suitable for 13C labelled small molecules?

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