I have seen different papers using different RBC concentrations (i,e, 0.5% and 1% chicke RBC) for HA and HI test ? Is there any specification when to use 0.5% or 1% RBC or can we use either of the concentration? Please clarify!
I am doing qPCR but my HPRT1 Ct is not coming at the same point. I see a 2 cycle spread in Ct. I have checked my RNA quality A260/280, 230/280 values, did ethidium bromide gel. I used superscript...
04 July 2024 5,605 3 View
While dealing with systematic classification we are following the standard taxonomic keys. A taxonomic key or guide gives us information about the identification, nomenclature, classification, and...
19 April 2024 9,054 0 View
I am working with leishmania threonine synthase (LdTS) enzyme of aspartic acid pathway which is ~75kDa protein. I purified the protein and confirmed by western blot too. The condition used for...
25 March 2024 7,978 3 View
Good Morning, i am going to perform cell wall loosening enzyme assays in wheat anyone who has done and want to share his/her protocol will be highly appreciated. My focus is on XET and expansins....
04 February 2024 9,963 0 View
Hey there, currently I am working on threonine synthase enzyme of Leishmania. I have successfully cloned it into E.coli DH5alpha strain. I have also transformed it into E.coli BL21(DE3) strain. I...
02 February 2024 1,962 4 View
02 February 2024 6,431 3 View
I performed an experiment involving the knockout (KO) of XYZ gene in CD34+ cells, followed by their differentiation into MKs. I am now seeking a reliable protocol for sorting the KO cells from the...
30 January 2024 8,319 0 View
I have about 40 draft genomes binned from metagenomes. I want to see their secondary metabolite production potentials. I used AntiSMASH in the command line. From the output files, I can see the...
29 January 2024 9,711 0 View
Hi, I extracted total cellular RNA from HEK293T cells by using a commercial kit. I left samples untreated or treated with 2ul RNaseA/T1 mixture (invitrogen) in the presence of PBS or protein...
11 January 2024 2,362 4 View
Is it possible to estimate the fluid flow behavior in the weld pool by analyzing the temperature distribution in the fusion zone?
27 December 2023 1,654 3 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
need to open an account to upload my published papers
01 August 2024 9,255 1 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....
31 July 2024 7,771 5 View
The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.
31 July 2024 10,109 3 View
I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me
30 July 2024 3,743 0 View
we have few papers ready for submission, and we need one co-author for each article who can pay article fee. Interested authors may text here or contact me on my following email id [email protected]
29 July 2024 6,626 0 View