Can anyone give me a protocol for cell viability assay using propidium iodide by FACS?

I am testing whether a knockdown causes cell death and want to look at the cell viability using propidium iodide, which I can analyze by FACS. Does anyone have a detailed protocol for this?

19 April 2017 1,575 2 View

I am looking for statistical analysis program that do analysis for 2 factor at 3 level, full factorial design, and also do optimization?

I am looking for statistical analysis program that do analysis for 2 factor at 3 level full factorial design, and also do optimization. We tried ANOVA univariate analysis for our experiment but...

26 November 2016 8,746 15 View

In RSM, If lack of fit comes significant then what should be the next step?

09 February 2016 8,945 6 View

How might I build a graph of connected components of an image?

31 May 2015 3,053 3 View

How to convert .nii into 2d image

have a file with .nii extension. I don't know how to convert a .nii file into 2D format.My question is while converting .nii file into 2D, am I losing some information about the file.Which format...

14 April 2015 1,666 14 View

Can any one suggest some study material for "impact of substitution in alloys on its electronic band structure".

Electronic band structure

17 December 2012 3,743 6 View

Unethical Practices in Paper Publication

One of my IEEE Conference paper has been copied end to end ..i was shocked to see this. Is there any way it can be pulled out. Very upset about these authors K THIRUPATHI RAO, M CHIRANJEEVI,...

01 January 1970 8,368 10 View

Where can i get free access to psychology scales for an experiment?

Hello, Can someone help me get access to the following scales (Free copy is available) 1. The Social Responsiveness Scale 2. The Vineland Adaptive Behavior Scales II (VABS). I am unable to...

01 January 1970 3,170 1 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

What kind of tape can be used to fix the samples on an XRD machine?

I have a very thin sample, the thickness is around 1 mm. I want to do X-ray diffraction measurement on the thin side of the sample. What kind of tape can I use to fix the sample, and will not...

13 February 2021 4,153 3 View