As we know vitronectin and some other proteins can bind to different types of integrin. I want to know about the binding site in Integrin which the vitronectin with a RGD-sequence can interact with and attach. I'll be thankful if any one help me.
Hi Razvan,
There is redundancy in the binding of alpha and beta integrins to Vitronectin. You can find which integrins (about which ones there is data) bind to it you can search Vitronectin in the String database:
http://string-db.org/newstring_cgi/show_input_page.pl?UserId=_8c9uaGLkaBR&sessionId=qwW4NDStZMDX
Which integrin / integrin alpha beta couple are you interested in?
Hi Dr. LLian,
Thanks a lot for introducing me the wonderful database.
Actually I want to know about the exact site in integrin (Alpha v Beta 3 or Alpha v Beta 5) which the vitronectin binds to. I know vitronectin binds with its RGD sequence, but I don't know the sequence of integrin that is in interface of binding. Is there any experimental results or I have to do some docking?
Hi Razvan, I have published on integrin avb3/5 binding to vitronectin.
Cellular av integrins do not bind to a vitronectin mutant that has the RGD sequence mutated to RGE.
If there is an alternative (non-RGD) integrin binding site on vitronectin, then it is not supporting cell adhesion through the avb3/5 integrins.
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Hi Dr Steingrimur,
Thanks for your answer but I have another paper which have reported the basic domain of vitronectin (heparin-binding domain of vitronectin) which does not have RGD can bind to integrin alpha V beta 5 (tested by affinity chromatography) as well as basic domain of Tat protein.
I'm wondering if you know anything about that and I'll be thankful let me know.
Hi Razvan, I am familiar with that paper. It is interesting that the RGD sequence in the Tat protein does not support cell adhesion, but the basic region does.
However, since cells attach, but do not spread on the Tat protein, indicates that the cytoskeleton is not engaged upon binding of avb5 to Tat.
Maybe cells bind weakly to a RGE-vitronectin mutant protein, but are washed off.
http://www.jbc.org/content/268/13/9725.full.pdf
http://www.sciencedirect.com/science/article/pii/S0006291X83714543
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