What is the indicated bands in small scale protein purification using Ni-NTA resin?

04 April 2017 0 4K Report

I am trying to purify DsRed protein from plasmid pET22-GEX6P2-mDsRed-2A10scFv which is E.coli derived. My protein is insoluble and the expected size is 76 kDa. When I am trying to purify my protein using Ni-NTA resin I got the expected band in case of both 10mM and 200mM imidazole/urea lane.

But I have also got another unexpected bands in all the lane. What is these bands?

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