What is the ideal method to sterilize glucose base media ?

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Elwood K Walls

You can simply make an aqueous solution of glucose at or above the final concentration for glucose and autoclave this solution before you mix the other components for the media. then add sterile water to reach required concentration for glucose after you have mixed other components.

Dr. Walls

Sumesh Kumar Dash

Thank you Sir.

Yijian Qiu

An easy option is filter sterilization with 0.2 micron sterile filter in the hood.