Can the stability test in Gaussian-DFT give indication to the global minimum point?

I am performing the frequency calculation for some of my molecules, and gradually optimized the structures till the final optimization using the basis set 6-311+G(d,p) and [opt=tight], then...

31 December 2018 5,527 4 View

A mixture of DMSO, WATER and ETHER. What are the effects of miscibility on material purification?

I am trying to extract my products from DMSO without using the evaporation method. I tried mixing with water, then ether, and succeeded in the purification by filtration; this method has been...

31 December 2018 3,914 8 View

A problem with seeding fibroblasts in collagen hydrogel?

Previously, I used to seed primary dermal fibroblasts (which initially had slow growth rate) in collagen hydrogel and the cells used to show complete attachment and the normal shape of fibroblasts...

09 October 2018 9,242 5 View

Why did the protein concentration of a hemolysate increase?

I am trying to purify a stroma-free lysed RBCs for hemoglobin isolation using 14 KDa dialysis bags. Using BCA assay for total protein quantification before the SDS-PAGE, I got the following...

02 March 2018 1,553 4 View

What may be the problem with these chromatograms?

I am analysing isolated heme from blood using HPLC (Mobile phase: Methanol:H2O: Acetic acid) with identification at two different wavelengths. The chromatograms at 405 and 500 nm are shown. I...

02 March 2018 7,346 16 View

Bradford assay or BCA assay for protein quantification?

Following the digestion of decellularized tissue using pepsin, which is a better method for the quantifying the released proteins to estimate the rate of reaction; Bradford assay or the BCA assay?

10 November 2017 3,216 3 View

How can we concentrate a partitioned protein solution?

Using PEG/salt aqueous two phase extraction system, after the partitioning of protein to either the polymer or salt phase, which are the suitable methods for concentrating of these proteins? Any...

09 October 2017 877 15 View

Can the extracellular matrix from bovine urinary bladder be used for hydrogel synthesis?

I am trying to make a hydrogel from the bovine Urinary Bladder Matrix (UBM), but can't get any hydrogel from the digested matrix using pepsin. Despite the complete digestion of both 10 and 20 %...

09 October 2017 3,345 2 View

What is the problem with this agarose gel electrophoresis image?

I am studying the release of DNA from nanoparticles in acidic medium at different times (lanes 4,5,6,7,8 from the left), but no bands appear in the gel in contrary to the main DNA appeared in...

04 May 2017 2,346 34 View

Should I ask for reproduction permission if an article is distributed under the Creative Commons Attribution License?

I am preparing a report and want to use some figures from some papers, and wish to know if I have to take the permission of the author, the journal or the publisher before using it as it is. Some...

11 December 2016 9,238 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

How to resolve Gaussian error termination " Error termination via Lnk1e in F:\G16W\l103.exe" for TS(QST3)?

When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...

21 February 2021 3,198 2 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View